Fingerprinting<i>Saccharomyces cerevisiae</i>Strains Using Next Generation Sequencing of PCR Amplicons Generated from Delta Elements

Identifying beer spoilage microbes is readily accomplished using PCR analyses targeting specific types of microbes, but the general classification wild yeast versus brewing cultivation independent molecular methods has remained challenging. The approach presented in this study utilized genetic fingerprint matching to determine if an unknown isolate matches catalogued strains. Interdelta Next Generation Sequencing (NGS) fingerprints were produced amplification delta elements, also known as long terminal repeat sequences transposons yeast. Fingerprints identifying different strains generated by processing reads NGS interdelta amplicons open source software. comprises DNA that can be recorded, compared, and a fixed reference for strain identification. shown reproducible capable distinguishing between Experimental contamination demonstrated utility in-house cross foreign contamination.